Based on epifluorescence microscopy
The three steps of FPR are: 1) measure fluorescence in a small region; 2) photobleach that region; and, 3) observe the return of fluorescence to reveal details of chemical recovery and, most importantly, molecular diffusion, which can be linked to polymer mass.
Blue light in, green light out
We can help you build FPR on your own microscope, perform a service for you, or deliver a turn-key system. FPR is only slightly more complicated than fluorescence correlation spectroscopy and has considerable advantages for molecular weight distribution studies.
No need for dust-free samples!
FPR is far more "nimble" than standard gel permeation chromatography (GPC). You can measure at high temperatures in 1 molar salt, then switch to pure water or even other solvents almost instantly. Here, we show a dialysis cell to switch salt environments in seconds.